Webb25 aug. 2024 · The sheared genomic DNA was used to prepare the SMRTbell library by SMRTbell sequencing adapters (Pacific Biosciences) following the manufacturer’s recommendations, and an approximately 10-kb insert library was sequenced on one SMRT cell using standard methods at Shanghai Majorbio Bio-Pharm Technology Co., Ltd.

Complete genome sequence of a novel chlorobenzene degrader,

Webb28 juli 2024 · Purified amplicons were pooled in equimolar amounts and paired-end sequenced on an Illumina MiSeq PE300 platform/NovaSeq PE250 platform (Illumina, San Diego, CA, USA) according to the standard protocols of Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China). Webb18 nov. 2024 · Raw data were processed according to the standard protocols provided by Majorbio Bio-Pharm Technology Co. Ltd. (Shanghai, China) as previously described ( Liu et al., 2024; Wang et al., 2024 ). In short, raw sequencing data was first de-multiplexed. cicd with github actions

Oral bacteria colonize and compete with gut microbiota in

Webb1 sep. 2024 · The 16S rRNA gene PCR amplification process was as follows: initial denaturation at 95 °C for 3 min, then followed by 27 cycles of denaturing at 95 °C for 30 s, annealing at 55 °C for 30 s and extension at 72 °C for 45 s, and single extension at 72 °C for 10 min, and end at 4 °C. WebbThen, we sent them to Shanghai Majorbio Bio-Pharm Technology Co., Ltd., China, for metagenomic sequencing (Illumina HiSeq sequencing). According to the manufacturer’s instructions, we extracted total genomic DNA from the composting samples using the E.Z.N.A. ® Soil DNA Kit (Omega Bio-Tek, Norcross, GA, USA). Webb16 okt. 2024 · All analyses were performed using the free Majorbio Cloud Platform (www.majorbio.com accessed on 6 December 2024), from Shanghai Majorbio Bio-pharm Technology Co., Ltd. The complete genome sequence was assembled using both PacBio and Illumina reads. cic ecommoy